"2×Speed Pfu PCR SuperMix (-dye ) is a ready-to-use mixture of Speed Pfu DNA polymerase, dNTPs, and optimized buffer. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2O.
• 2×Speed Pfu PCR SuperMix (-dye ) offers 108-fold fidelity as compared to DNA Polymerase.
• Extension rate is about 2-6 kb/min.
• PCR products can be directly cloned into pEASY-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb." https://molecular-tools.cr...

Creative Biolabs is dedicated to providing several viable and cost-effective methods for pluripotent stem cells (iPSC) reprogramming. We employ advanced iPSC reprogramming factor delivery strategy by virus, iPSC reprogramming factor delivery by episomal vectors, as well as other iPSC reprogramming methods (mRNA, protein) to help you obtaining the desired iPSC. Learn more, https://www.creative-biola...

Our QVirus™ platform can offer the packaging of your CRISPR libraries into our lentiviral vectors. CRISPR lentiviral libraries are packaged into highly pure and concentrated lentiviral particles, either from an existing lentiviral plasmid library or from a custom plasmid library that we construct starting from a provided oligonucleotide pool, corresponding to your guide sequences.

https://qvirus.creative-bi...

The Calculus & Vectors Cheat Sheet You Never Knew You Needed

What do you think of when you hear the words calculus and vectors? Complicated mathematical equations? Frustration from high school math classes? Fear of getting a low grade?

https://www.uscaacademy.co...

What are prerequisites to start learning machine learning?

To start learning machine learning, it's helpful to have a foundation in certain prerequisite skills and knowledge areas. Here are some key prerequisites to consider:

Mathematics:
Understanding of basic mathematics concepts, including algebra, calculus, probability, and statistics. Linear algebra is particularly important for understanding machine learning algorithms and concepts such as matrix operations, eigenvalues, and eigenvectors.
Programming:
Proficiency in at least one programming language, such as Python, R, or Julia. Python is widely used in the machine learning community and has extensive libraries and frameworks for machine learning and data science, such as NumPy, pandas, scikit-learn, TensorFlow, and PyTorch.

A unique suspension of HEK293 and HEK293T cell lines cultured without the use of animals has been created. To produce adenoviruses and lentiviruses, cell lines that have been quickly optimized for the scalable production of all serotypes and adeno-associated virus chimeras (AAV) can also be created.

High-density adherent cell culture bioreactors are used to increase adhesion to HEK293 and HeLa culture procedures. Viral vectors with high titers can be generated in batch mode by transfection or infection techniques. The production and upstream process are improved by the bioreactor system.
For more: https://www.creative-biola...

Empowering Your Math Skills: The Importance of Calculus and Vectors

Remember the time when math seemed like a subject confined to the four walls of a classroom, quickly forgotten once the bell rang? If you're currently navigating your way through the challenging MCV4U course, you might be pondering the real-world relevance of mastering calculus and vectors. Look no further than the smartphone in your hand or the latest space exploration missions. From the technology that keeps you connected to the thrilling twists and turns of amusement park rides, calculus and vectors are always behind the scenes, making everything—from precise engineering feats to digital communications— possible.

https://www.uscaacademy.co...

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