Cells are structural and functional unit of our body which control and maintain the function of all unicellular and multicellular organisms. The process of cell proliferation and differentiation plays a key role from the time of embryogenesis to development of whole organism from single- or double- cell embryo and continues its critical role in maintenance of adult tissue homoeostasis by recycling the old cells with new cells. Besides, abnormal cell proliferation is also associated with human diseases like cancer. In sum, cell proliferation assays are very important in scrutinizing the rate of cell proliferation in both in vitro and in vivo conditions.

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