BSA is a widely used genetic mapping method to analyze genomic loci affecting a trait of interest. The technology usually mixes individuals with extreme traits in a separated group to build two mixed pools, then the molecular markers closely linked to the target gene can be quickly located by analyzing the frequency difference of alleles between the two mixed pools. BSA overcomes the limitation of obtaining near-isogenic lines in many crops, thus it can save a lot of time. It is a very practical method for gene marker positioning.
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